The goal of our research is to quantitatively understand human RNA regulatory networks and their dysregulation in human disease. RNA metabolism is a function of multiple interdependent regulatory processes orchestrated by ribonucleoprotein complexes (RNPs). RNPs are dynamic regulatory interactions between RNA-binding proteins (RBPs) and RNAs that determine the fate of an RNA. Systematic analysis of RNA regulation with methods that quantitatively measure each step in RNA metabolism is necessary to understand pathophysiological changes in gene regulatory networks. We employ quantitative, transcriptome-wide techniques to map physical interactions as well as RNA regulatory events. We combine experimental and computational approaches to investigate normal and aberrant RNA regulation in the context of human steroidogenesis and cancer.